中文名称

人乳头瘤病毒18型E4抗体

别    名

HPV18 E4 protein; HPV 18 E4 protein;   Human Papilloma Virus; Human papillomavirus type 18 E4; Human papillomavirus   type 18 E4; Protein E4; Human Papillomavirus 18 (E4); HPV18 E4; E4 protein   (HPV18); VE4_HPV18; HPV18-E4.  

抗体来源

Rabbit

克隆类型

Polyclonal

产品应用

IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
  not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user.

理论分子量

10kDa

细胞定位

细胞核 

性    状

Liquid

浓    度

1mg/ml

免 疫 原

KLH conjugated synthetic peptide   derived from HPV18 E4: 31-88/88 

亚    型

IgG

纯化方法

affinity purified by Protein A

缓 冲 液

0.01M TBS(pH7.4) with 1% BSA, 0.03%   Proclin300 and 50% Glycerol.

保存条件

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated   freeze/thaw cycles.

注意事项

This product as supplied is intended   for research use only, not for use in human, therapeutic or diagnostic   applications.

产品介绍

The two primary oncoproteins of high   risk HPV types are E6 and E7. The “E” designation indicates that these two   proteins are expressed early in the HPV life cycle, while the "L"   designation indicates late expression. The HPV genome is composed of six   early (E1, E2, E4, E5, E6, and E7) ORFs, two late (L1 and L2) ORFs, and a   non-coding long control region (LCR). After the host cell is infected viral   early promoter is activated and a polycistronic primary RNA containing all   six early ORFs is transcribed. This polycistronic RNA then undergoes active   RNA splicing to generate multiple isoforms of mRNAs. One of the spliced   isoform RNAs, E6*I, serves as an E7 mRNA to translate E7 protein. However,   viral early transcription subjects to viral E2 regulation and high E2 levels   repress the transcription. HPV genomes integrate into host genome by   disruption of E2 ORF, preventing E2 repression on E6 and E7. Thus, viral   genome integration into host DNA genome increases E6 and E7 expression to   promote cellular proliferation and the chance of malignancy. The degree to   which E6 and E7 are expressed is correlated with the type of cervical lesion   that can ultimately develop.