UDP葡萄糖神经酰胺葡萄糖基转移酶(UGCG)活性蛋白

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Lys39~Leu171

Tags: N-terminal His-tag

Purity: >95%

Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl

and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.7

Predicted Molecular Mass: 16.1kDa

Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions. Note: 98% cross-reactivity of UGCG was observed among human, mouse and rat

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. 

Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition. 

[ SEQUENCE ]

[ ACTIVITY ] 

UDP Glucose Ceramide Glucosyltransferase (UGCG) is an enzyme whichcatalyzes the first glycosylation step in glycosphingolipid biosynthesis. It belongsto the glycosyltransferase 2 family. UGCG is widely expressed and transcription isupregulated during keratinocyte differentiation. Besides, Large MultifunctionalPeptidase 2 (LMP2) has been identified as an interactor of UGCG, thus a bindingELISA assay was conducted to detect the interaction of recombinant humanUGCG and recombinant human LMP2. Briefly, UGCG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred toLMP2-coated microtiter wells and incubated for 2h at 37℃. Wells were washedwith PBST and incubated for 1h with anti-UGCG pAb, then aspirated and washed3 times. After incubation with HRP labelled secondary antibody, wells wereaspirated and washed 3 times. With the addition of substrate solution, wells wereincubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells andread at 450nm immediately. The binding activity of UGCG and LMP2 was shown inFigure 1, and this effect was in a dose dependent manner.

[ IDENTIFICATION ]

[ IMPORTANT NOTE ] 

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

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