可罗索(KL)活性蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Leu517~Leu956
Tags: N-terminal His-tag
Purity: >94%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 54.8kDa
Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[ SEQUENCE ]
[ ACTIVITY ]
Klotho (KL) is a transmembrane protein that, in addition to other effects, providessome control over the sensitivity of the organism to insulin and appears to beinvolved in aging. The Klotho protein is a novel β-glucuronidase capable ofhydrolyzing steroid β-glucuronides. Genetic variants in KLOTHO have beenassociated with human aging, and Klotho protein has been shown to be acirculating factor detectable in serum that declines with age. The binding of certainfibroblast growth factors (FGF's) viz., FGF19, FGF20, and FGF23, to theirfibroblast growth factor receptors, is promoted via their interactions asco-receptors with β-Klotho. Besides, Fibroblast Growth Factor 23 (FGF23) hasbeen identified as an interactor of KL, thus a binding ELISA assay was conductedto detect the interaction of recombinant rat KL and recombinant rat FGF23. Briefly, KL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of100μL were then transferred to FGF23-coated microtiter wells and incubated for2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-KL pAb,then aspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substratesolution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stopsolution to the wells and read at 450nm immediately. The binding activity of KL andFGF23 was shown in Figure 1, and this effect was in a dose dependent manner.
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考。