组织蛋白酶A(CTSA)活性蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Ala151~Val394
Tags: Two N-terminal Tags, His-tag and GST-tag
Purity: >92%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 7.0
Predicted Molecular Mass: 57.9kDa
Accurate Molecular Mass: 57kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[ SEQUENCE ]
[ ACTIVITY ]
Cathepsin A (CTSA) is an enzyme that is classified both as a cathepsin and acarboxypeptidase. This gene encodes a glycoprotein that associates withlysosomal enzymes beta-galactosidase and neuraminidase to form a complex ofhigh-molecular-weight multimers. The formation of this complex provides aprotective role for stability and activity. It is protective for β-galactosidase andneuraminidase. Besides, Neuraminidase (NEU) has been identified as aninteractor of CTSA, thus a binding ELISA assay was conducted to detect theinteraction of recombinant rat CTSA and recombinant rat NEU. Briefly, CTSA werediluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µL werethen transferred to NEU-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CTSA pAb, thenaspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substratesolution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stopsolution to the wells and read at 450nm immediately. The binding activity of CTSAand NEU was shown in Figure 1, and this effect was in a dose dependent manner.
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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