Slit同源物2(Slit2)活性蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Asn209~Gly374
Tags: N-terminal His-tag
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.8
Predicted Molecular Mass: 19.9kDa
Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[ SEQUENCE ]
[ ACTIVITY ]
Slit is a family of secreted extracellular matrix proteins which play an importantsignalling role in the neural development of most bilaterians. Humans, mice andother vertebrates possess three Slit homologs: Slit1, Slit2 and Slit3. The Slit2protein has recently been discovered to be associated with the development ofnew blood vessels from pre-existing vessels, or angiogenesis. Slit2 has beenimplicated in promoting angiogenesis in mice (both in vitro and in vivo), in thehuman placenta, and in tumorigenesis. Besides, Gremlin 1 (GREM1) has beenidentified as an interactor of Slit2, thus a binding ELISA assay was conducted todetect the interaction of recombinant rat Slit2 and recombinant rat GREM1. Briefly, Slit2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of100uL were then transferred to GREM1-coated microtiter wells and incubated for2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Slit2 pAb,then aspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substratesolution, wells were incubated 15-25 minutes at 37 ℃ . Finally, add 50µL stopsolution to the wells and read at 450nm immediately. The binding activity of Slit2and GREM1 was shown in Figure 1, and this effect was in a dose dependentmanner.
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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