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碳酸酐酶Ⅱ(CA2)活性蛋白
[ PROPERTIES ]
Source: Natural Extract
Host: Rat (Erythrocytes)
Purity: >98%
Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl
and 5% trehalose. Applications: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.9
Predicted Molecular Mass: 29kDa
Accurate Molecular Mass: 29kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[ ACTIVITY ]
CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration ofcarbon dioxide. It is essential for bone resorption and osteoclast differentiation andcontributes to intracellular pH regulation in the duodenal upper villous epitheliumduring proton-coupled peptide absorption. It is widely accepted that CA2 alsocatalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay wasconducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA)as substrate. Briefly, different concentrations of CA2 were incubated with 1mM4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was readper hour, and the result was shown in figure 1. It is obvious that CA2 catalyzeshydrolysis of p-Nitrophenyl Acetate.
[ IDENTIFICATION ]
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