Rabbit Anti-Chicken IgM / RBITC

Quantity size:

100ul  1ml 

Concentration:

2mg/ml   Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.

Also known as:

Rabbit Anti-Chicken IgM (RBITC); Immunoglobulin M;

Specificity:

Rabbit Polyclonal IgG, affinity purified by Protein A.

Reacts with: Chicken, .

Immunogen: Native Chicken IgM.

Storage:

10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Application:

IF=1:100-1000  
Excitation spectrum: 540nm
Emission spectrum: 625nm
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.

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