Rabbit Anti-Mouse Kappa light chain / RBITC

Quantity size:

100ul  1ml 

Concentration:

2mg/ml   Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

Also known as:

Rabbit Anti-Mouse Kappa light chain (RBITC)

Specificity:

Rabbit Polyclonal IgG, affinity purified by Protein A.

Reacts with: Mouse, .

Immunogen: KLH conjugated synthetic peptide derived from Mouse Kappa light chain.

Storage:

10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Application:

IF=1:100-1000  
Excitation spectrum: 540nm
Emission spectrum: 625nm
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.

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