Biotinylated Human SCF / KITLG Protein, Avitag™,His Tag
分子别名(Synonym)
KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL
表达区间及表达系统(Source)
Biotinylated Human SCF, Avitag,His Tag is expressed from human 293 cells . It contains AA Glu 26 - Ala 190 .
Predicted N-terminus: Glu 26
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 21.7 kDa. The protein migrates as 26-38 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
标记(Biotinylation)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Biotin:Protein Ratio)
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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