NR0B2 Polyclonal Antibody

Host: Rabbit   Reactivity:Human,Mouse,Rat

BackGround:

The protein encoded by this gene is an unusual orphanreceptor that contains a putative ligand-binding domainbut lacks a conventional DNA-binding domain. The geneproduct is a member of the nuclear hormone receptorfamily, a group of transcription factors regulated by smallhydrophobic hormones, a subset of which do not haveknown ligands and are referred to as orphan nuclear receptors.The protein has been shown to interact with retinoidand thyroid hormone receptors, inhibiting their ligand-dependenttranscriptional activation. In addition, interactionwith estrogen receptors has been demonstrated,leading to inhibition of function. Studies suggest that theprotein represses nuclear hormone receptor-mediatedtransactivation via two separate steps: competition withcoactivators and the direct effects of its transcriptionalrepressor function.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide,50% glycerol, pH7.2

Molecular Weight:

~ 28 kDa

Swiss-Prot:

Q15466

Purification&Purity:

The antibody was affinity-purified from rabbit antiserumby affinity-chromatography using epitope-specific immunogenand the purity is > 95% (by SDS-PAGE).

Applications:

WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C longterm. Avoid freeze-thaw cycles.

Specificity:

NR0B2 polyclonal antibody detects endogenous levels ofNR0B2 protein.

DATA:

The NF-κB/Rel transcription factors are present in thecytosol in an inactive state complexed with the inhibitoryIκB proteins. Activation occurs via phosphorylation ofIκBα at Ser32 and Ser36 followed by proteasome-mediateddegradation that results in the releaseand nuclear translocation of active NF-κB. IκBα phosphorylationand resulting Rel-dependent transcription areactivated by a highly diverse group of extracellular signalsincluding inflammatory cytokines, growth factors,and chemokines. Kinases that phosphorylate IκB at theseactivating sites have been identified. The regulation ofIκBβ and IκBε is similar to that of IκBα. However, thephosphorylation and ubiquitin-mediated degradation ofthese proteins occurs with much slower kinetics. IKKphosphorylation of IκBβ occurs at Ser19 and Ser23, whileIκBε can be phosphorylated at Ser18 and Ser22.