心肌肌钙蛋白I(cTnI)活性蛋白

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Met1~Gly203

Tags: N-terminal His-tag

Purity: >98%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose. Applications: Cell culture; Activity Assays. 

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.7

Predicted Molecular Mass: 24.4kDa

Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions. 

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 

2. Relative charge: The composition of amino acids may affects the charge of the protein. 

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. 

Store at 2-8^oC for one month. 

Aliquot and store at -80^oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 ^oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. 

[ SEQUENCE ]

[ ACTIVITY ]

Troponin I Type 3, Cardiac (TNNI3) is a protein that in humans is encoded by the TNNI3 gene. It is a tissue-specific subtype of troponin I, which in turn is a part of the troponin complex. The TNNI3 gene encoding cardiac troponin I (cTnI) is located at 19q13.4 in the human chromosomal genome. Human cTnI is a 24kDa protein consisting of 210 amino acids with isoelectric point (pI) of 9.87. cTnI is exclusively expressed in adult cardiac muscle. Besides, Troponin T Type 2, Cardiac (TNNT2) has been identified as an interactor of TNNI3, thus a binding ELISA assay was conducted to detect the interaction of recombinant humanTNNI3 and recombinant human TNNT2. Briefly, TNNI3 was diluted serially in PBSwith 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred toTNNT2-coated microtiter wells and incubated for 2h at 37℃. Wells were washedwith PBST and incubated for 1h with anti-TNNI3 pAb, then aspirated and washed 3times. After incubation with HRP labelled secondary antibody, wells were aspiratedand washed 3 times. With the addition of substrate solution, wells were incubated15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at450nm immediately. The binding activity of TNNI3 and TNNT2 was shown inFigure 1, and this effect was in a dose dependent manner.

[ IDENTIFICATION ]

Antibody: Rabbit Anti-Human TNNI3 Ab

[ IMPORTANT NOTE ] 

The kit is designed for in vitro and research use only, we will not be responsiblefor any issue if the kit was used in clinical diagnostic or any other procedures.

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