phospho-RACGAP1 (Ser387) Polyclonal Antibody

Host: Rabbit  Reactivity:Human,Mouse,Rat,Dog,Cow,

BackGround:

Rho GTPases control a variety of cellular processes.There are 3 subtypes of Rho GTPases in the Ras superfamilyof small G proteins: RHO, RAC and CDC42.GTPase-activating proteins (GAPs) bind activated formsof Rho GTPases and stimulate GTP hydrolysis. Throughthis catalytic function, Rho GAPs negatively regulateRho-mediated signals. GAPs may also serve as effectormolecules and play a role in signaling downstream ofRho and other Ras-like GTPases.

Product:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and50% Glycerol.

Molecular Weight:

71kDa

Swiss-Prot:

Q9H0H5

Purification&Purity:

affinity purified by Protein A

Applications:

WB=1:500-2000

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C longterm. Avoid freeze-thaw cycles.

Specificity:

phospho-RACGAP1 (Ser387) Polyclonal Antibody detectsendogenous levels of RACGAP1 alpha proteinonly when phosphorylated at Ser387.

DATA:

Primary: Anti-phospho-RACGAP1 (Ser387) at 1/1000 dilution

Note:

For research use only, not for use in diagnostic procedure.

The human retinoid X receptors (RXRs) are encoded bythree distinct genes (RXRα, RXRβ, and RXRγ) and bindselectively and with high affinity to the vitamin A derivative,9-cis-retinoic acid. RXRs are type-II nuclear hormonereceptors that are largely localized to the nuclearcompartment independent of ligand binding. NuclearRXRs form heterodimers with nuclear hormone receptorsubfamily 1 proteins, including thyroid hormone receptor,retinoic acid receptors, vitamin D receptor, peroxisomeproliferator-activated receptors, liver X receptors, andfarnesoid X receptor. Since RXRs heterodimerize withmultiple nuclear hormone receptors, they play a centralrole in transcriptional control of numerous hormonal signalingpathways by binding to cis-acting response elementsin the promoter/enhancer region of target genes.Retinoid X receptor α (RXRα) is the founding RXR familymember and is predominantly expressed in liver, kidney,epidermis, intestine and a variety of tissues. Knockoutof the murine rxrα gene results in embryonic lethalitytentatively due to myocardial hypoplasia, which demonstratesthe importance of RXRα to retinoid signaling invivo. Biochemical evidence suggests that RXRα transcriptionalactivity is post-translationally regulatedthrough the Ras-Raf-MAPK signaling cascade.MAPK-dependent phosphorylation of RXRα directly abrogatesthe ability of RXRα to associate with nuclear receptorcoactivators.