PARP2 Polyclonal Antibody

Host: Rabbit   Reactivity:Human,Mouse,Rat

BackGround:

Involved in the base excision repair (BER) pathway, bycatalyzing the poly(ADP-ribosyl)ation of a limited numberof acceptor proteins involved in chromatin architectureand in DNA metabolism. This modification followsDNA damages and appears as an obligatory step in a detection/signalingpathway leading to the reparation ofDNA strand breaks. Mediates serine ADP-ribosylation oftarget proteins following interaction with HPF1; HPF1conferring serine specificity.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide,50% glycerol, pH7.2

Molecular Weight:

~ 66 kDa

Swiss-Prot:

Q9UGN5

Purification&Purity:

The antibody was affinity-purified from rabbit antiserumby affinity-chromatography using epitope-specific immunogenand the purity is > 95% (by SDS-PAGE).

Applications:

WB:1:1000~1:2000

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C longterm. Avoid freeze-thaw cycles.

Specificity:

PARP2 polyclonal antibody detects endogenous levels ofPARP2 protein.

DATA:

Note:

For research use only, not for use in diagnostic procedure.

The NF-κB/Rel transcription factors are present in thecytosol in an inactive state complexed with the inhibitoryIκB proteins. Activation occurs via phosphorylation ofIκBα at Ser32 and Ser36 followed by proteasome-mediateddegradation that results in the releaseand nuclear translocation of active NF-κB. IκBα phosphorylationand resulting Rel-dependent transcription areactivated by a highly diverse group of extracellular signalsincluding inflammatory cytokines, growth factors,and chemokines. Kinases that phosphorylate IκB at theseactivating sites have been identified. The regulation ofIκBβ and IκBε is similar to that of IκBα. However, thephosphorylation and ubiquitin-mediated degradation ofthese proteins occurs with much slower kinetics. IKKphosphorylation of IκBβ occurs at Ser19 and Ser23, whileIκBε can be phosphorylated at Ser18 and Ser22.