VPS4A Polyclonal Antibody

Host: Rabbit   Reactivity:Human,Mouse,Rat

BackGround:

The protein encoded by this gene is a member of the AAAprotein family (ATPases associated with diverse cellularactivities), and is the homolog of the yeast Vps4 protein.In humans, two paralogs of the yeast protein have beenidentified. The former share a high degree of aa sequencesimilarity with each other, and also with yeast Vps4 andmouse Skd1 proteins. The mouse Skd1 (suppressor of K+transport defect 1) has been shown to be really an yeastVps4 ortholog. Functional studies indicate that both humanparalogs associate with the endosomal compartments,and are involved in intracellular protein trafficking,similar to Vps4 protein in yeast. The gene encodingthis paralog has been mapped to chromosome 16; thegene for the other resides on chromosome 18.

Product:

1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Molecular Weight:

~ 49kDa

Swiss-Prot:

Q9UN37

Purification&Purity:

The antibody was affinity-purified from rabbit antiserumby affinity-chromatography using epitope-specific immunogenand the purity is > 95% (by SDS-PAGE).

Applications:

WB: 1:500~1:2000

IHC: 1:50~1:200

IF: 1:50~1:200

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C longterm. Avoid freeze-thaw cycles.

Specificity:

VPS4A polyclonal antibody detects endogenous levels ofVPS4A protein.

DATA:

Note:

For research use only, not for use in diagnostic procedure.

The NF-κB/Rel transcription factors are present in thecytosol in an inactive state complexed with the inhibitoryIκB proteins. Activation occurs via phosphorylation ofIκBα at Ser32 and Ser36 followed by proteasome-mediateddegradation that results in the releaseand nuclear translocation of active NF-κB. IκBα phosphorylationand resulting Rel-dependent transcription areactivated by a highly diverse group of extracellular signalsincluding inflammatory cytokines, growth factors,and chemokines. Kinases that phosphorylate IκB at theseactivating sites have been identified. The regulation ofIκBβ and IκBε is similar to that of IκBα. However, thephosphorylation and ubiquitin-mediated degradation ofthese proteins occurs with much slower kinetics. IKKphosphorylation of IκBβ occurs at Ser19 and Ser23, whileIκBε can be phosphorylated at Ser18 and Ser22.