TOMM20 Rabbit monoclonal Antibody

Host: Rabbit   Reactivity:Human,Mouse,Rat

BackGround:

The mitochondrial preprotein translocases of the outermembrane (Tom) is a multisubunit protein complex thatfacilitates the import of nucleus-encoded precursor proteinsacross the mitochondrial outer membrane. The Tommachinery consists of import receptors for the initialbinding of cytosolically synthesized preproteins and ageneral import pore (GIP) for the membrane translocationof various preproteins into the mitochondria. The importreceptors include Tom20 and Tom22, which form a heteromericreceptor complex that initiates the insertion ofnewly synthesized proteins into the outer membrane andthen directs the precursor protein into the GIP. In yeast,Tom22 is the essential component of the import receptorcomplex as it functions as both a receptor for the preproteinsand serves as a docking point for both Tom20 andthe GIP.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide,50% glycerol, pH7.2

Molecular Weight:

~ 16 kDa

Swiss-Prot:

Q15388

Purification&Purity:

Protein A affinity purified

Applications:

WB: 1:1000-1:2000

IHC/ICC/IF: 1:50-1:200

FC: 1:50-1:100

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C longterm. Avoid freeze-thaw cycles.

Specificity:

This antibody detects endogenous levels of TOMM20 anddoes not cross-react with related proteins.

DATA:

Note:

For research use only, not for use in diagnostic procedure.

The NF-κB/Rel transcription factors are present in thecytosol in an inactive state complexed with the inhibitoryIκB proteins. Activation occurs via phosphorylation ofIκBα at Ser32 and Ser36 followed by proteasome-mediateddegradation that results in the releaseand nuclear translocation of active NF-κB. IκBα phosphorylationand resulting Rel-dependent transcription areactivated by a highly diverse group of extracellular signalsincluding inflammatory cytokines, growth factors,and chemokines. Kinases that phosphorylate IκB at theseactivating sites have been identified. The regulation ofIκBβ and IκBε is similar to that of IκBα. However, thephosphorylation and ubiquitin-mediated degradation ofthese proteins occurs with much slower kinetics. IKKphosphorylation of IκBβ occurs at Ser19 and Ser23, whileIκBε can be phosphorylated at Ser18 and Ser22.