RPS27A polyclonal antibody

Host: Rabbit   Reactivity:Human,Mouse,Rat

BackGround:

Ubiquitin, a highly conserved protein that has a majorrole in targeting cellular proteins for degradation by the26S proteosome, is synthesized as a precursor proteinconsisting of either polyubiquitin chains or a single ubiquitinfused to an unrelated protein. This gene encodes afusion protein consisting of ubiquitin at the N terminusand ribosomal protein S27a at the C terminus. When expressedin yeast, the protein is post-translationally processed,generating free ubiquitin monomer and ribosomalprotein S27a. Ribosomal protein S27a is a component ofthe 40S subunit of the ribosome and belongs to theS27AE family of ribosomal proteins. It contains C4-typezinc finger domains and is located in the cytoplasm.Pseudogenes derived from this gene are present in thegenome. As with ribosomal protein S27a, ribosomal proteinL40 is also synthesized as a fusion protein with ubiquitin;similarly, ribosomal protein S30 is synthesized as afusion protein with the ubiquitin-like protein fubi. Multiplealternatively spliced transcript variants that encode thesame proteins have been identified.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide,50% glycerol, pH7.2

Molecular Weight:

~ 14 kDa

Swiss-Prot:

P62979

Purification&Purity:

The antibody was affinity-purified from rabbit antiserumby affinity-chromatography using epitope-specific immunogenand the purity is > 95% (by SDS-PAGE).

Applications:

WB 1:500 - 1:2000

IF 1:50 - 1:200

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C longterm. Avoid freeze-thaw cycles.

Specificity:

RPS27A polyclonal antibody detects endogenous levelsof RPS27A protein.

DATA:

Note:

For research use only, not for use in diagnostic procedure.