Ribosomal Protein S27L (E14) polyclonal antibody

Host: Rabbit   Reactivity:Human,Mouse,Rat

BackGround:

One way that growth factors and mitogens effectivelypromote sustained cell growth and proliferation is by upregulatingmRNA translation. Growth factors and mitogensinduce the activation of p70 S6 kinase and the subsequentphosphorylation of the S6 ribosomal protein.Phosphorylation of S6 ribosomal protein correlates withan increase in translation of mRNA transcripts that containan oligopyrimidine tract in their 5' untranslated regions.These particular mRNA transcripts (5'TOP) encodeproteins involved in cell cycle progression, as well as ribosomalproteins and elongation factors necessary fortranslation. Important S6 ribosomal protein phosphorylationsites include several residues (Ser235, Ser236,Ser240, and Ser244) located within a small, carboxy-terminalregion of the S6 protein

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide,50% glycerol, pH7.2

Molecular Weight:

~ 11 kDa

Swiss-Prot:

Q71UM5

Purification&Purity:

The antibody was affinity-purified from rabbit antiserumby affinity-chromatography using epitope-specific immunogenand the purity is > 95% (by SDS-PAGE).

Applications:

WB: 1:500~1:1000

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C longterm. Avoid freeze-thaw cycles.

Specificity:

Ribosomal Protein S27L (E14) polyclonal antibody endogenouslevels of Ribosomal Protein S27L.

DATA:

Western blot (WB) analysis of Ribosomal Protein S27L (E14) polyclonal

antibody at 1:500 dilution

Lane1:The Kidney tissue lysate of Mouse(40ug)

Lane2:The Prostate tissue lysate of Rat(40ug)

Lane3:SGC7901 whole cell lysate(40ug)

Lane4:Panc1 whole cell lysate(40ug)

Note:

For research use only, not for use in diagnostic procedure.