A-Raf (phospho-Y302) polyclonal antibody

Host: Rabbit   Reactivity: Human,Mouse,Rat

BackGround:Several serine/threonine protein kinases have been implicatedas intermediates in signal transduction pathways.These include ERK/MAP kinases, ribosomal S6 kinase(Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsicserine/threonine activity. It is broadly expressed innearly all cell lines tested to date and is the cellular homologof v-Raf, the product of the transforming gene of the3611 strain of murine sarcoma virus. The unregulated kinaseactivity of the v-Raf protein has been associated withtransformation and mitogenesis while the activity ofRaf-1 is normally suppressed by a regulatory N-terminaldomain. A-Raf, a second member of the Raf gene familyof serine/ threonine protein kinases, exhibits substantialhomology to Raf-1 within the kinase domain of the twomolecules, but less homology elsewhere. Expression ofA-Raf is found at highest levels in urogenital tissues andkidney and at lowest level in brain tissue.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide,50% glycerol, pH7.2

Molecular Weight:

~ 68 kDa

Swiss-Prot:

P10398

Purification&Purity:

The antibody was affinity-purified from rabbit antiserumby affinity-chromatography using epitope-specific immunogen

and the purity is > 95% (by SDS-PAGE).

Applications:

WB: 1:500~1:1000

IHC: 1:50~1:200

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C longterm. Avoid freeze-thaw cycles.

Specificity:

p-A-Raf (Y302) polyclonal antibody detects endogenouslevels of A-Raf protein only when phosphorylated atTyr302.

DATA:

Note:

For research use only, not for use in diagnostic procedure.